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Predicted assignments of cells to donor from iPSCs assignment of cell identify to donors using default settings in demuxlet14

Journal: NAR Genomics and Bioinformatics

Article Title: Comparative performance of the BGI and Illumina sequencing technology for single-cell RNA-sequencing

doi: 10.1093/nargab/lqaa034

Figure Lengend Snippet: Predicted assignments of cells to donor from iPSCs assignment of cell identify to donors using default settings in demuxlet14

Article Snippet: iPSC and TMWC libraries were sequenced on an Illumina NextSeq 500 (NextSeq control software v2.0.2/Real Time Analysis v2.4.11) using a 150 cycle NextSeq High Output Reagent Kit v2.5 (Illumina: 20024907) in stand-alone mode as follows: 26 bp (Read 1), 8 bp (Index) and 98 bp (Read 2).

Techniques:

Experiment-specific metrics. ( A ) Metrics related to guide RNA assignment in TMWC. This excludes cells that were not affiliated with a guide RNA and cells that with ambiguous assignments. ( B ) Number of SNPs called per cell in iPSCs. SNPs were called from alignments of cells found in NextSeq 500 and MGISEQ-2000 datasets.

Journal: NAR Genomics and Bioinformatics

Article Title: Comparative performance of the BGI and Illumina sequencing technology for single-cell RNA-sequencing

doi: 10.1093/nargab/lqaa034

Figure Lengend Snippet: Experiment-specific metrics. ( A ) Metrics related to guide RNA assignment in TMWC. This excludes cells that were not affiliated with a guide RNA and cells that with ambiguous assignments. ( B ) Number of SNPs called per cell in iPSCs. SNPs were called from alignments of cells found in NextSeq 500 and MGISEQ-2000 datasets.

Article Snippet: iPSC and TMWC libraries were sequenced on an Illumina NextSeq 500 (NextSeq control software v2.0.2/Real Time Analysis v2.4.11) using a 150 cycle NextSeq High Output Reagent Kit v2.5 (Illumina: 20024907) in stand-alone mode as follows: 26 bp (Read 1), 8 bp (Index) and 98 bp (Read 2).

Techniques: